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HGF Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG2a

Detection: Mouse / IgG1

Reactivity

Capture: Human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.313-20 ng/mL

Cat No : MP50034-1

Print datasheet

Synonyms

F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF

Validation Data Gallery

  • Sandwich ELISA standard curve of MP50034-1, HGF Monoclonal Matched Antibody Pair - PBS only. 68660-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1084. 68660-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

    Sandwich ELISA standard curve of MP50034-1

    Sandwich ELISA standard curve of MP50034-1, HGF Monoclonal Matched Antibody Pair - PBS only. 68660-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard HZ-1084. 68660-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP50034-1 targets HGF in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.313-20 ng/mL (Sandwich ELISA);

Capture antibody: HGF Monoclonal antibody, PBS Only (Capture) 68660-1-PBS (2G2C3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: HGF Monoclonal antibody, PBS Only (Detector) 68660-2-PBS (2E5G2). 100 μg. Concentration 1 mgl/ml.

Alternative HGF matched antibody pairs: MP00454-1, MP00454-2

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 68660-1-PBS
Tested Reactivity Human
Immunogen HGF fusion protein HZ-1084
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name hepatocyte growth factor (hepapoietin A; scatter factor)
Synonyms F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF
Gene symbol HGF
Gene ID (NCBI) 3082
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 68660-2-PBS
Tested Reactivity Human
Immunogen HGF fusion protein HZ-1084
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hepatocyte growth factor (hepapoietin A; scatter factor)
Synonyms F TCF, Hepatocyte growth factor, Hepatopoeitin A, HGF, HGFB, HPTA, Scatter factor, SF
Gene symbol HGF
Gene ID (NCBI) 3082
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.