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(-)-Blebbistatin

(-)-Blebbistatin ((S)-(-)-Blebbistatin) 是 blebbistatin 的 S 对映异构体。它是一种有效的选择性肌球蛋白 II 抑制剂，IC50 范围为 0.5 至 5 μM。

CAS号

856925-71-8

分子式

C18H16N2O2

主要靶点

Myosin|ATPase

仅限科研使用

Cat No : CM05239

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Synonyms

(S)-(-)-Blebbistatin

验证数据展示

  • CAS No.: 856925-71-8

    (-)-Blebbistatin
    CAS#: 856925-71-8

产品信息

(-)-Blebbistatin is an S enantiomer of blebbistatin. It is a potent and selective myosin II inhibitor with IC50 ranging from 0.5 to 5 μM.

CAS号 856925-71-8
分子式 C18H16N2O2
主要靶点 Myosin|ATPase
主要通路 细胞骨架|离子通道
分子量 292.33
纯度 99.59%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 (S)-(-)-Blebbistatin

靶点活性

non-muscle myosin II ATPases:0.5 μM-5 μM

体内活性

Blebbistatin completely relaxes both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction in a dose-dependent manner. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz[4].

溶解度

DMSO:29.2 mg/mL (100 mM)

细胞实验

Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed[3].

参考文献

1.Straight AF, et al. Science, 2003, 299(5613), 1743-1747. 2.Limouze J, et al. J Muscle Res Cell Motil, 2004, 25(4-5), 337-341. 3.Kovács M, et al. J Biol Chem, 2004, 279(34), 35557-35563 4.Zhang X, et al. In vitro and in vivo relaxation of urinary bladder smooth muscle by the selective myosin IIinhibitor, blebbistatin. BJU Int. 2011 Jan;107(2):310-7. 5.Tian X, Gao M, Li A, et al. Protocol for Isolation of Viable Adult Rat Cardiomyocytes with High Yield[J]. STAR Protocols. 2020: 100045.

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The dilution calculator equation

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