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14-3-3 Sigma Polyclonal antibody, PBS Only

14-3-3 Sigma Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10622-1-PBS

Print datasheet

Synonyms

SFN, HME1, 14-3-3sigma, 14-3-3 protein sigma

验证数据展示

  • WB analysis using 10622-1-AP (same clone as 10622-1-PBS)

    WB analysis using 10622-1-AP (same clone as 10622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

    IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
  • IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

    IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
  • IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

    IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10622-1-AP (14-3-3 Sigma antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
  • IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

    IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10622-1-AP (14-3-3 Sigma antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
Planning an IHC experiment? We recommend our IHCeasy SFN Ready-To-Use IHC Kit. SFN primary antibody included.

产品信息

10622-1-PBS targets 14-3-3 Sigma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen 14-3-3 Sigma fusion protein Ag0973 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name stratifin
Synonyms SFN, HME1, 14-3-3sigma, 14-3-3 protein sigma
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-30 kDa
GenBank Accession NumberBC002995
Gene Symbol 14-3-3 Sigma
Gene ID (NCBI) 2810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31947
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.