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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 10622-1-AP (same clone as 10622-1-PBS)

    WB analysis using 10622-1-AP (same clone as 10622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

    IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
  • IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

    IHC staining of mouse skin using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 10622-1-AP (14-3-3 sigma antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
  • IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

    IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10622-1-AP (14-3-3 Sigma antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included
  • IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

    IHC staining of human bowen disease using 10622-1-AP (same clone as 10622-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 10622-1-AP (14-3-3 Sigma antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10622-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SFN antibody included

14-3-3 Sigma Polyclonal antibody, PBS Only

14-3-3 Sigma Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10622-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

SFN, HME1, 14-3-3sigma, 14-3-3 protein sigma

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SFN Ready-To-Use IHC Kit. SFN primary antibody included.

产品信息

10622-1-PBS targets 14-3-3 Sigma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 14-3-3 Sigma fusion protein Ag0973 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stratifin
别名 SFN, HME1, 14-3-3sigma, 14-3-3 protein sigma
计算分子量 28 kDa
观测分子量 28-30 kDa
GenBank蛋白编号BC002995
基因名称 14-3-3 Sigma
Gene ID (NCBI) 2810
RRIDAB_2254479
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31947
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

14-3-3 Sigma is a member 14-3-3 family of highly conserved proteins participating in diverse cellular processes including cell cycle progression. It is exclusively present in various epithelial cells, and alternatively named as human epithelial marker (HEM), or stratifin. Alteration of its expression has been linked to cancer.