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14-3-3E Monoclonal antibody, PBS Only

14-3-3E Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A3A4

Cat no : 66946-1-PBS

Print datasheet

Synonyms

14 3 3 protein epsilon, 14 3 3E, 14-3-3E, FLJ45465, KCIP 1, MDCR, MDS, YWHAE

验证数据展示

  • WB analysis of HeLa using 66946-1-Ig (same clone as 66946-1-PBS)

    WB analysis of HeLa using 66946-1-Ig (same clone as 66946-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66946-1-Ig (14-3-3E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

    IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66946-1-Ig (14-3-3E antibody) at dilution of 1:1 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAE antibody included
  • IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

    IHC staining of rat liver using 66946-1-Ig (same clone as 66946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66946-1-Ig (14-3-3E antibody) at dilution of 1:1 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66946-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YWHAE antibody included
Planning an IHC experiment? We recommend our IHCeasy YWHAE Ready-To-Use IHC Kit. YWHAE primary antibody included.

产品信息

66946-1-PBS targets 14-3-3E in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen 14-3-3E fusion protein Ag25186 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide
Synonyms 14 3 3 protein epsilon, 14 3 3E, 14-3-3E, FLJ45465, KCIP 1, MDCR, MDS, YWHAE
Calculated Molecular Weight 255 aa, 29 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC000179
Gene Symbol 14-3-3E
Gene ID (NCBI) 7531
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62258
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

14-3-3 epsilon (also known as YWHAE) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.