2-Deoxy-D-glucose

2-Deoxy-D-glucose (2-DG) 是葡萄糖的类似物,是一种具有抗病毒活性的糖酵解抑制剂。

CAS号

154-17-6

分子式

C6H12O5

主要靶点

Hexokinase|Apoptosis|HSV

仅限科研使用

Cat No : CM00910

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Synonyms

D-Arabino-2-deoxyhexose|2-Deoxy-D-arabino-hexose|2-deoxyglucose|2-DG|Ba 2758|D-2-脱氧葡萄糖|NSC 15193|2-脱氧-D-葡萄糖|2-DG



产品信息

2-Deoxy-D-glucose is an analog of glucose, which is a glycolytic inhibitor with antiviral activity.

CAS号 154-17-6
分子式 C6H12O5
主要靶点 Hexokinase|Apoptosis|HSV
主要通路 代谢|微生物学|凋亡
分子量 164.16
纯度 99.31%, 此纯度可做参考,具体纯度与批次有关系,可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 D-Arabino-2-deoxyhexose|2-Deoxy-D-arabino-hexose|2-deoxyglucose|2-DG|Ba 2758|D-2-脱氧葡萄糖|NSC 15193|2-脱氧-D-葡萄糖|2-DG

体内活性

Treatment of cancer patients with relatively high doses of 2-DG (greater than 200 mg/kg) was largely ineffective in managing tumor growth. Side effects of 2-DG included elevated blood glucose levels, progressive weight loss with lethargy, and behavioral symptoms of hypoglycemia[2]. 2-DG enhances isoflurane-induced loss of righting reflex in mice. By reducing metabolism, 2-DG treatment can decrease body temperature in rodent, enhancing sensitivity to anesthetics[3]. 2-DG diet significantly increased serum ketone body level and brain expression of enzymes required for ketone body metabolism. The 2-DG-induced maintenance of mitochondrial bioenergetics was paralleled by simultaneous reduction in oxidative stress. Further, 2-DG treated mice exhibited a significant reduction of both amyloid precursor protein (APP) and amyloid beta (Aβ) oligomers, which was paralleled by significantly increased α-secretase and decreased γ-secretase expression, indicating that 2-DG induced a shift towards a non-amyloidogenic pathway. 2-DG increased expression of genes involved in Aβ clearance pathways, degradation, sequestering, and transport. Concomitant with increased bioenergetic capacity and reduced β-amyloid burden, 2-DG significantly increased expression of neurotrophic growth factors, BDNF and NGF, thus reduces pathology in female mouse model of Alzheimer's disease[4].

体外活性

2-Deoxy-D-glucose(2-DG) activates AKT function through phosphatidylinositol 3-kinase (PI3K) and is independent of glycolysis or mTOR inhibition. 2-DG treatments disrupts the binding between insulin-like growth factor 1 (IGF-1) and IGF-binding protein 3 (IGFBP3) so that the free form of IGF-1 could be released from the IGF-1·IGFBP3 complex to activate IGF-1 receptor (IGF1R) signaling. 2-DG-induced activation of many survival pathways can be jointly attenuated through IGF1R inhibition. 2-DG also induces time- and dose-dependent ERK phosphorylation[1]. 2-DG is readily transported into cells and is phosphorylated by hexokinase, but cannot be metabolized further and accumulates in the cell. This leads to ATP depletion and the induction of cell-death[2]. 2DG significantly suppresses proliferation, causes apoptosis and reduces migration of murine endothelial cells, inhibiting formation of lamellipodia and filopodia and causing disorganization of F-actin filaments in murine endothelial cell[5].

溶解度

H2O:16.4 mg/mL (100 mM),DMSO:16.4 mg/mL (100 mM)

细胞实验

2×103 H460 or H157 cells are seeded in 96-well cell culture plates. Cells are treated with 5 mM 2-DG only, 5 or 10 μM IGF1R inhibitor II only, or a combination of 2-DG and IGF1R inhibitor II. Cell growth inhibition is determined after 48 h by the CellTiter 96® AQueous nonradioactive cell proliferation assay. (Only for Reference)

参考文献

1.Zhong D, et al. J Biol Chem. 2009, 284(35):23225-33.
2.Marsh J, et al. Nutr Metab (Lond). 2008, 5:33.
3.Wang H, et al. Anesth Analg. 2015, 120(2):312-9.
4.Yao J, et al. PLoS One. 2011, 6(7):e21788.
5.Huang CC, et al. Dis Model Mech. 2015, 8(10):1247-54.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
×
=
×
C1   V1   C2   V2