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PPP3CC-specific Polyclonal antibody

PPP3CC-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55163-1-AP

Print datasheet

Synonyms

PPP3CC, CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3

验证数据展示

  • WB analysis of fetal human brain using 55163-1-AP

    WB analysis of fetal human brain using 55163-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 55163-1-AP (PPP3CC-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 55163-1-AP

    IF Staining of U2OS using 55163-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human stomach using 55163-1-AP

    IHC staining of human stomach using 55163-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 55163-1-AP (PPP3CC-specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected infetal human brain tissue, human brain tissue, human testis tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55163-1-AP targets PPP3CC-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform
Synonyms PPP3CC, CNA3, CAM-PRP catalytic subunit, CAM PRP catalytic subunit, CALNA3
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberNM_005605
Gene Symbol PPP3CC
Gene ID (NCBI) 5533
RRIDAB_10860906
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48454
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP3CC, also named as CALNA3 and CNA3, belongs to the PPP phosphatase family and PP-2B subfamily. It is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CC.

实验方案

Product Specific Protocols
WB protocol for PPP3CC-specific antibody 55163-1-APDownload protocol
IHC protocol for PPP3CC-specific antibody 55163-1-APDownload protocol
IF protocol for PPP3CC-specific antibody 55163-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Acta Biomater

Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.

Authors - Hong Cao
View Article
ratIHC

BMC Pregnancy Childbirth

Core biomarkers analysis benefit for diagnosis on human intrahepatic cholestasis of pregnancy

Authors - Yan Fang
View Article