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3BDO

3BDO 是新型的mTOR 激活剂，对自噬有抑制作用。

CAS号

890405-51-3

分子式

C18H17NO5

主要靶点

mTOR|Autophagy|Apoptosis

仅限科研使用

Cat No : CM03090

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Synonyms

验证数据展示

  • CAS No.: 890405-51-3

    3BDO
    CAS#: 890405-51-3

产品信息

3BDO is a new mTOR activator. 3BDO inhibits autophagy.

CAS号 890405-51-3
分子式 C18H17NO5
主要靶点 mTOR|Autophagy|Apoptosis
主要通路 自噬|凋亡|PI3K/Akt/mTOR信号通路
分子量 327.33
纯度 96.00%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名

体内活性

Immunofluorescence assay reveals that 3BDO treatment increases the level of p-p70S6K and decreases the protein level of ATG13 in plaque endothelium of mice. 3BDO does not affect the phosphorylation of mTOR direct downstream targets p70S6K and 4EBP1. As compare with controls, apoE-/- mice show inhibited endothelium autophagy and apoptosis with 3BDO treatment, so 3BDO protects against endothelium injury in atherosclerosis. 3BDO treatment stabilizes established atherosclerotic lesions in apoE-/- mice. In apoE-/-mice, as compare with controls, with 3BDO treatment, the serum level of IL-6 and IL-8 is significantly decreased[2].

体外活性

Phosphorylation of RPS6KB1 and EIF4EBP1 is significantly increased by 3BDO with vector alone but suppressed with FKBP1A overexpression. Rapamycin fails to decrease the phosphorylation of MTOR and RPS6KB1 in the presence of 3BDO. 3BDO suppresses the increase in MAP1LC3B puncta induced with rapamycin. 3BDO also inhibits the effect of rapamycin in HUVECs. The phosphorylation of Ser residues is decreased in HUVECs treated with 10 μM rapamycin, and 60 μM 3BDO reverses the phosphorylation. The results show that 3BDO suppresses the increased MAP1LC3B puncta number, MAP1LC3B-II level and decreased SQSTM1 protein level induced by rapamycin. 3BDO could dose- and time-dependently decrease FLJ11812 level in HUVECs. Overexpression of FLJ11812 reverses the inhibition of autophagy induced by 3BDO[1].

溶解度

Ethanol:20 mg/mL (61.10 mM),Sonification is recommended.,DMSO:150 mg/mL (458.25 mM)

参考文献

1.Ge D, et al. Identification of a novel MTOR activator and discovery of a competing endogenous RNA regulating autophagy in vascular endothelial cells. Autophagy. 2014 Jun;10(6):957-71.
2.Peng N, et al. An activator of mTOR inhibits oxLDL-induced autophagy and apoptosis in vascular endothelial cells and restricts atherosclerosis in apolipoprotein E?/? mice. Sci Rep. 2014 Jul 1;4:5519.

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