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  • Featured Product
  • KD/KO Validated

4-1BBL/TNFSF9 Monoclonal antibody, PBS Only (Capture)

4-1BBL/TNFSF9 Monoclonal Antibody for WB, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B4B1

Cat no : 66450-1-PBS

Print datasheet

Synonyms

TNFSF9, CD137L, 4-1BB ligand, 4 1BBL, 4 1BB ligand

验证数据展示

  • WB analysis of Raji using 66450-1-Ig (same clone as 66450-1-PBS)

    WB analysis of Raji using 66450-1-Ig (same clone as 66450-1-PBS)

    Raji, Ramos, Daudi, and K-562 cells were subjected to SDS PAGE followed by western blot with 66450-1-Ig (TNFSF9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66450-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50011-1

    Sandwich ELISA standard curve of MP50011-1

    Sandwich ELISA standard curve of MP50011-1, 4-1BBL/TNFSF9 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66450-1-PBS. Detection antibody: HRP-conjugated 66450-2-PBS. Standard: Ag12768. Range: 0.313-20 ng/mL.

产品信息

66450-1-PBS targets 4-1BBL/TNFSF9 as part of a matched antibody pair:

MP50011-1: 66450-1-PBS capture and 66450-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity human
Immunogen 4-1BBL/TNFSF9 fusion protein Ag12768 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor (ligand) superfamily, member 9
Synonyms TNFSF9, CD137L, 4-1BB ligand, 4 1BBL, 4 1BB ligand
Calculated Molecular Weight 254 aa, 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC104805
Gene Symbol TNFSF9
Gene ID (NCBI) 8744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP41273
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TNFSF9, also named as 4-1BBL, belongs to the tumor necrosis factor family. It is a cytokine that binds to TNFRSF9. TNSF9 induces the proliferation of activated peripheral blood T-cells. It may have a role in activation-induced cell death (AICD) and may play a role in cognate interactions between T-cells and B-cells/macrophages.