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验证数据展示

  • WB analysis using 84185-5-RR

    WB analysis using 84185-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84185-5-RR (4-1BBL/TNFSF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Raji using 84185-5-RR

    IF Staining of Raji using 84185-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using 4-1BBL/TNFSF9 antibody (84185-5-RR, Clone: 241386E7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

4-1BBL/TNFSF9 Recombinant antibody

4-1BBL/TNFSF9 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84185-5-RR

产品说明书

CloneNo. 241386E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

TNFSF9, CD137L, 4-1BB ligand, 4 1BBL, 4 1BB ligand

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells, K-562 cells, HEK-293T cells
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84185-5-RR targets 4-1BBL/TNFSF9 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor necrosis factor (ligand) superfamily, member 9
别名 TNFSF9, CD137L, 4-1BB ligand, 4 1BBL, 4 1BB ligand
计算分子量 254 aa, 27 kDa
观测分子量35 kDa
GenBank蛋白编号BC104805
基因名称 TNFSF9
Gene ID (NCBI) 8744
RRIDAB_3671740
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP41273
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNFSF9, also named as 4-1BBL, belongs to the tumor necrosis factor family. It is a cytokine that binds to TNFRSF9. TNSF9 induces the proliferation of activated peripheral blood T-cells. It may have a role in activation-induced cell death (AICD) and may play a role in cognate interactions between T-cells and B-cells/macrophages.

实验方案

Product Specific Protocols
WB protocol for 4-1BBL/TNFSF9 antibody 84185-5-RRDownload protocol
IF protocol for 4-1BBL/TNFSF9 antibody 84185-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols