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验证数据展示

  • WB analysis using 81812-4-RR (same clone as 81812-4-PBS)

    WB analysis using 81812-4-RR (same clone as 81812-4-PBS)

    Non-treated HEK-293T cells, phosphatase inhibitor treated HEK-293T cells and λ phosphatase treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 81812-4-RR (Phospho-4EBP1 (Thr37) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81812-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00559-2

    Cytometric bead array standard curve of MP00559-2

    Cytometric bead array standard curve of MP00559-2, Phospho-4EBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81812-4-PBS. Detection antibody: 81812-3-PBS. Standard: SY01767P. Range: 0.78-50 ng/mL.

Phospho-4EBP1 (Thr37) Recombinant antibody, PBS Only (Capture)

Phospho-4EBP1 (Thr37) Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA
Cat No. 81812-4-PBS

产品说明书

CloneNo. 240535G3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Cytometric bead array, Indirect ELISA

别名

EIF4EBP1, eIF4E binding protein 1, BP 1, 4E-BP1, 4EBP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EIF4EBP1 Ready-To-Use IHC Kit. EIF4EBP1 primary antibody included.

产品信息

81812-4-PBS targets Phospho-4EBP1 (Thr37) as part of a matched antibody pair:

MP00559-2: 81812-4-PBS capture and 81812-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E binding protein 1
别名 EIF4EBP1, eIF4E binding protein 1, BP 1, 4E-BP1, 4EBP1
计算分子量 118 aa, 12 kDa
观测分子量 15-20 kDa
GenBank蛋白编号BC004459
基因名称 EIF4EBP1
Gene ID (NCBI) 1978
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13541
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)