• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 83890-5-RR (same clone as 83890-5-PBS)

    WB analysis using 83890-5-RR (same clone as 83890-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83890-5-RR (4EBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83890-5-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83890-5-RR (same clone as 83890-5-PBS)

    FC experiment of HeLa using 83890-5-RR (same clone as 83890-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug 4EBP1 Recombinant antibody (83890-5-RR, Clone:241055E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83890-5-PBS in a different storage buffer formulation.

  • FC experiment of HEK-293 using 83890-5-RR (same clone as 83890-5-PBS)

    FC experiment of HEK-293 using 83890-5-RR (same clone as 83890-5-PBS)

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug 4EBP1 Recombinant antibody (83890-5-RR, Clone:241055E7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83890-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83890-5-RR

    Affinity and Kinetic Characterization of 83890-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83890-5-RR against Human 4EBP1 were performed. The affinity constant is 0.752 nM.

4EBP1 Recombinant antibody, PBS Only

4EBP1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83890-5-PBS

产品说明书

CloneNo. 241055E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

EIF4EBP1, eIF4E-binding protein 1, eIF4E binding protein 1, BP 1, 4E-BP1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy EIF4EBP1 Ready-To-Use IHC Kit. EIF4EBP1 primary antibody included.

产品信息

83890-5-PBS targets 4EBP1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 4EBP1 fusion protein Ag5056 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 4E binding protein 1
别名 EIF4EBP1, eIF4E-binding protein 1, eIF4E binding protein 1, BP 1, 4E-BP1
计算分子量 118 aa, 12 kDa
观测分子量15-20 kDa
GenBank蛋白编号BC004459
基因名称 EIF4EBP1
Gene ID (NCBI) 1978
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13541
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827)