5-Carboxylcytosine (5-caC) antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Not Species Specific

Applications

DB, ICC, IF

Cat No : 61229,61230 61229

Synonyms

5-carboxylcytidine, 5-carboxylcytosine, 5-fC, 5fc, TET, dna methylation, carboxylcytosine, stem cell, stem cells, embryogenesis, 5-cac, 5cac, sample



产品信息

Tested Applications DB, ICC, IF

Applications Validated by Active Motif: DB: 0.5 - 2 ug/ml dilution

Tested Reactivity Not Species Specific
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
ModificationMethylated
Immunogen This 5-Carboxylcytosine antibody was raised against 5-carboxylcytidine conjugated to KLH and recognizes 5-carboxylcytosine.
Full Name 5-Carboxylcytosine (5-caC) antibody (pAb)
Synonyms 5-carboxylcytidine, 5-carboxylcytosine, 5-fC, 5fc, TET, dna methylation, carboxylcytosine, stem cell, stem cells, embryogenesis, 5-cac, 5cac, sample
Molecular weight
GenBank accession numberN/A
RRIDAB_2793559
Purification Method Protein A Chromatography
Buffer Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.