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5-Ethynyluridine

5-Ethynyluridine 可用于标记新合成的 RNA。这种方法称为使用点击化学 (RICK) 捕获新转录的 RNA 相互作用组，系统地捕获与多种 RNA 结合的蛋白质，包括新生 RNA 和传统上被忽视的非多腺苷酸化 RNA。

CAS号

69075-42-9

分子式

C11H12N2O6

主要靶点

Nucleoside Antimetabolite/Analog

仅限科研使用

Cat No : CM01976

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Synonyms

验证数据展示

  • CAS No.: 69075-42-9

    5-Ethynyluridine
    CAS#: 69075-42-9

产品信息

5-Ethynyluridine can be used to label newly synthesized RNA. This approach, named capture of the newly transcribed RNA interactome using click chemistry (RICK), systematically captures proteins bound to a wide range of RNAs, including nascent RNAs and traditionally neglected nonpolyadenylated RNAs.

CAS号 69075-42-9
分子式 C11H12N2O6
主要靶点 Nucleoside Antimetabolite/Analog
主要通路 DNA损伤和修复|细胞周期
分子量 268.22
纯度 100.00%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名

体内活性

EU labelling can be used to examine transcription in vivo in mouse models of nervous system disorders. Injection of EU directly into the cerebellum results in reproducible labelling of newly transcribed RNA in cerebellar neurons and glia, with cell type-specific differences in relative labelling intensities, such as Purkinje cells exhibiting the highest levels. EU-labelling accumulating into cytoplasmic inclusions, indicating that EU, like other modified uridines, may introduce non-physiological properties in labelled RNAs[1].

溶解度

PBS (pH 7.2):5 mg/mL,DMSO:10 mg/mL,DMF:10 mg/mL

参考文献

1.Van't Sant LJ, et al. In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration. Acta Neuropathol Commun. 2021 May 21;9(1):94.
2.Bao X, et al. Capturing the interactome of newly transcribed RNA. Nat Methods. 2018 Mar;15(3):213-220.

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