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验证数据展示

  • WB analysis using 10021-1-Ig (same clone as 10021-1-PBS)

    WB analysis using 10021-1-Ig (same clone as 10021-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10021-1-Ig (5 Lipoxygenase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 10021-1-Ig (same clone as 10021-1-PBS)

    IF Staining of HeLa using 10021-1-Ig (same clone as 10021-1-PBS)

    Immunofluorescent analysis of Hela cells, using ALOX5 antibody 10021-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10021-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

5 Lipoxygenase Polyclonal antibody, PBS Only

5 Lipoxygenase Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 10021-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

10021-1-PBS targets 5 Lipoxygenase in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 arachidonate 5-lipoxygenase
别名
计算分子量 674 aa, 78 kDa
观测分子量 70-78 kDa
GenBank蛋白编号BC130332
基因名称 5 Lipoxygenase
Gene ID (NCBI) 240
RRIDAB_513237
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP09917
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).