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5 Lipoxygenase Monoclonal antibody, PBS Only

5 Lipoxygenase Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B1B7

Cat No : 66326-1-PBS

Print datasheet

Synonyms

5 lipoxygenase, 5 LO, 5 LOX, 5LPG, ALOX5, arachidonate 5 lipoxygenase, LOG5

验证数据展示

  • WB analysis of pig spleen using 66326-1-Ig (same clone as 66326-1-PBS)

    WB analysis of pig spleen using 66326-1-Ig (same clone as 66326-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 66326-1-Ig (same clone as 66326-1-PBS)

    WB analysis of THP-1 using 66326-1-Ig (same clone as 66326-1-PBS)

    WB result of 5 Lipoxygenase antibody (66326-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-5 Lipoxygenase transfected THP-1 cells. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 66326-1-Ig (same clone as 66326-1-PBS)

    WB analysis of THP-1 using 66326-1-Ig (same clone as 66326-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66326-1-Ig (5 Lipoxygenase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • WB analysis of pig spleen using 66326-1-Ig (same clone as 66326-1-PBS)

    WB analysis of pig spleen using 66326-1-Ig (same clone as 66326-1-PBS)

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66326-1-Ig (ALOX5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

    IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

    IHC staining of human lung using 66326-1-Ig (same clone as 66326-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66326-1-Ig (ALOX5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66326-1-PBS in a different storage buffer formulation.

产品信息

66326-1-PBS targets 5 Lipoxygenase in WB, IHC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, pig
Immunogen 5 Lipoxygenase fusion protein Ag18168 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name arachidonate 5-lipoxygenase
Synonyms 5 lipoxygenase, 5 LO, 5 LOX, 5LPG, ALOX5, arachidonate 5 lipoxygenase, LOG5
Calculated Molecular Weight 674 aa, 78 kDa
Observed Molecular Weight 78 kDa
GenBank Accession NumberBC130332
Gene Symbol 5 Lipoxygenase
Gene ID (NCBI) 240
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09917
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ALOX5(arachidonate 5-lipoxygenase) is also named as LOG5, 5-LO and belongs to the lipoxygenase family. It catalyzes the first step in leukotriene biosynthesis, and plays a role in inflammatory processes. It is a 78 kDa iron-containing enzyme that translocates from the cytosol to associate with FLAP, and converts arachidonate in two steps into the unstable intermediate LTA4. It is essential for cysteinyl-leukotriene (cys-LT) production, critical mediators in asthma(PMID:12911785).