AMPK:0.8 μM(EC50)

方法：雄性 BALB/c 小鼠腹腔注射 LPS 2 mg/kg，4 小时后腹腔注射 A-769662 10 mg/kg，8 小时后处死小鼠。 结果：A-769662 显著降低 Tlr-4 mRNA、MyD88 蛋白、TNF-α/IL-6 水平，同时增加 p-AMPK 和 p-ACC 水平。[2]

方法：小鼠 BMSCs 加入 10 μM A-769662，处理 6 天，分时间点检测，Western blot 检测 p-AMPKα （Thr172） 和 p-ACC （Ser79）。 结果：A-769662 显著增加 AMPKα及其底物 ACC 的磷酸化水平，确认激活 AMPK 信号。[1]

Ethanol:3.61 mg/mL (10.02 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:4.5 mg/mL (12.49 mM);DMSO:51 mg/mL (141.51 mM)

Cell viability of MEF cells treated or not with A-769662 is performed as follows: cells are harvested by trypsinization and incubated with 0.5 mg/mL RNase and 50 μg/mL propidium iodine at room temperature in the dark; cell viability is analyzed by flow cytometry using a FACScanto flow cytometer, using an excitation laser at 488 nm and a propidium iodine fluorescence detection at 600 nm. To determine the proportion of cells in each phase of the cell cycle, cells are harvested by trypsinization, collected by centrifugation, washed in PBS and fixed overnight in 80% ethanol at -20 °C. Subsequently, these fixed cells are centrifuged to remove the fixative and incubated for 20 minutes in the dark at room temperature in PBS containing 0.5 mg/mL RNase and 50 μg/mL propidium iodine. Flow cytometry analysis is performed as above. The proportion of cells in G1, S, and G2 is determined using the MODFIT program. Cell culture pictures are taken at the indicated times using a camera coupled to an inverted microscope with a 20 × objective. (Only for Reference)