验证数据展示
经过测试的应用
Positive WB detected in | Neuro-2a cells, mouse liver, mouse brain |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22647-1-AP targets A2BP1 in WB, IHC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF-P, IP, ELISA Application Description |
文献引用应用 | WB, IF, RIP |
经测试反应性 | human, mouse |
文献引用反应性 | human |
免疫原 | A2BP1 fusion protein Ag18527 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ataxin 2-binding protein 1 |
别名 | FOX1, RBFOX1, HRNBP1, Hexaribonucleotide-binding protein 1, Fox-1 homolog A |
计算分子量 | 395 aa, 42 kDa |
观测分子量 | 45-70 kDa |
GenBank蛋白编号 | BC113691 |
基因名称 | A2BP1 |
Gene ID (NCBI) | 54715 |
RRID | AB_2879142 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9NWB1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
A2BP1, also named as FOX1 and HRNBP1, contains one RRM (RNA recognition motif) domain. A2BP1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. A2BP1 and ataxin-2 are both localized in the trans-Golgi network. This is a rabbit polyclonal antibody raised against the N terminus of human A2BP1.A2BP1 has two mouse isoforms (A2BP1-A016 and -A030) exogenously expressed in COS7 cells showed a molecular mass of 65 kDa, although the calculated molecular masses of A016 and A030 are 40 and 42 kDa, respectively in the course of cell biological study.
实验方案
Product Specific Protocols | |
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WB protocol for A2BP1 antibody 22647-1-AP | Download protocol |
IHC protocol for A2BP1 antibody 22647-1-AP | Download protocol |
IF protocol for A2BP1 antibody 22647-1-AP | Download protocol |
IP protocol for A2BP1 antibody 22647-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Mol Ther Oncolytics RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway.
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MedComm (2020) Single-cell transcriptomics reveal the remodeling landscape of bladder in patients with obstruction-induced detrusor underactivity |