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验证数据展示

  • WB analysis using 22647-1-AP

    WB analysis using 22647-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22647-1-AP (A2BP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse brain using 22647-1-AP

    IF Staining of mouse brain using 22647-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse brain using 22647-1-AP

    IP experiment of mouse brain using 22647-1-AP

    IP result of anti-A2BP1 (IP:22647-1-AP, 4ug; Detection:22647-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • IHC staining of mouse brain using 22647-1-AP

    IHC staining of mouse brain using 22647-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22647-1-AP (A2BP1 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with RBFOX1/A2BP1 antibody included
  • IF Staining of mouse brain using 22647-1-AP

    IF Staining of mouse brain using 22647-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 22647-1-AP

    IF Staining of mouse brain using 22647-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using A2BP1 antibody (22647-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

A2BP1 Polyclonal antibody

A2BP1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 22647-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, IP, ELISA and More (2)

Synonyms

FOX1, RBFOX1, HRNBP1, Hexaribonucleotide-binding protein 1, Fox-1 homolog A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inNeuro-2a cells, mouse liver, mouse brain
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy RBFOX1/A2BP1 Ready-To-Use IHC Kit. RBFOX1/A2BP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22647-1-AP targets A2BP1 in WB, IHC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IF, RIP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen A2BP1 fusion protein Ag18527 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ataxin 2-binding protein 1
Synonyms FOX1, RBFOX1, HRNBP1, Hexaribonucleotide-binding protein 1, Fox-1 homolog A
Calculated Molecular Weight 395 aa, 42 kDa
Observed Molecular Weight 45-70 kDa
GenBank Accession NumberBC113691
Gene Symbol A2BP1
Gene ID (NCBI) 54715
RRIDAB_2879142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWB1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

A2BP1, also named as FOX1 and HRNBP1, contains one RRM (RNA recognition motif) domain. A2BP1 recognizes a (U)GCAUG stretch in regulated exons or in flanking introns. The protein binds to the C-terminus of ataxin-2 and may contribute to the restricted pathology of spinocerebellar ataxia type 2 (SCA2). Ataxin-2 is the product of the SCA2 gene which causes familial neurodegenerative diseases. A2BP1 and ataxin-2 are both localized in the trans-Golgi network. This is a rabbit polyclonal antibody raised against the N terminus of human A2BP1.A2BP1 has two mouse isoforms (A2BP1-A016 and -A030) exogenously expressed in COS7 cells showed a molecular mass of 65 kDa, although the calculated molecular masses of A016 and A030 are 40 and 42 kDa, respectively in the course of cell biological study.

实验方案

Product Specific Protocols
WB protocol for A2BP1 antibody 22647-1-APDownload protocol
IHC protocol for A2BP1 antibody 22647-1-APDownload protocol
IF protocol for A2BP1 antibody 22647-1-APDownload protocol
IP protocol for A2BP1 antibody 22647-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,RIP

Mol Ther Oncolytics

RBFOX1 Regulates the Permeability of the Blood-Tumor Barrier via the LINC00673/MAFF Pathway.

Authors - Shuyuan Shen
  • KD Validated
View Article
humanIF

MedComm (2020)

Single-cell transcriptomics reveal the remodeling landscape of bladder in patients with obstruction-induced detrusor underactivity

Authors - Jiawei Chen
View Article