• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

A4GNT Polyclonal antibody

A4GNT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22670-1-AP

Print datasheet

Synonyms

EC:2.4.1.-, Alpha4GnT, Alpha-1,4-N-acetylglucosaminyltransferase

验证数据展示

  • WB analysis using 22670-1-AP

    WB analysis using 22670-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22670-1-AP (A4GNT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 22670-1-AP

    IHC staining of human stomach cancer using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 22670-1-AP

    IHC staining of human stomach cancer using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 22670-1-AP (A4GNT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach using 22670-1-AP

    IHC staining of human stomach using 22670-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 22670-1-AP (A4GNT antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inK-562 cells, mouse pancreas tissue, mouse stomach tissue, rat stomach tissue, L02 cells
Positive IHC detected inhuman stomach cancer tissue, human pancreas cancer tissue, rat stomach tissue, mouse stomach tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22670-1-AP targets A4GNT in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen A4GNT fusion protein Ag18432 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alpha-1,4-N-acetylglucosaminyltransferase
Synonyms EC:2.4.1.-, Alpha4GnT, Alpha-1,4-N-acetylglucosaminyltransferase
Calculated Molecular Weight 340 aa, 39 kDa
Observed Molecular Weight 34-37 kDa
GenBank Accession NumberBC119639
Gene Symbol A4GNT
Gene ID (NCBI) 51146
RRIDAB_11183768
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNA3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

A4GNT (alpha-1,4-N-acetylglucosaminyltransferase) catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans, forming a unique glycan structure, GlcNAcalpha1-->4Galbeta-->R. The gene is expressed with a bias in certain tissues, particularly in the stomach (RPKM 9.5) and duodenum (RPKM 0.9). Alpha4GnT has been implicated in O-glycan biosynthesis and is thought to have a role in preventing gastric cancer by inhibiting Helicobacter pylori infection and suppressing tumor-promoting inflammation.

实验方案

Product Specific Protocols
WB protocol for A4GNT antibody 22670-1-APDownload protocol
IHC protocol for A4GNT antibody 22670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols