验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SH-SY5Y cells, NIH/3T3 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
15127-1-AP targets ALADIN in WB, IF, IHC, ELISA applications and shows reactivity with human, monkey, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, monkey, mouse |
| 文献引用反应性 | human |
| 免疫原 | ALADIN fusion protein Ag7211 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | achalasia, adrenocortical insufficiency, alacrimia (Allgrove, triple-A) |
| 别名 | AAA, AAAS, AAASb, ADRACALA, ADRACALIN, ALADIN, GL003 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 56-60 kDa |
| GenBank蛋白编号 | BC000659 |
| 基因名称 | AAAS |
| Gene ID (NCBI) | 8086 |
| RRID | AB_2219477 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NRG9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The AAAS gene product is the 60 kDa nuclear pore complex (NPC) protein alacrima-achalasia-adrenal insufficiency neurological disorder (ALADIN). AAAS mRNA and the ALADIN protein are ubiquitously expressed with predominance in the adrenal and central nervous system structures in human and rat. ALADIN, a Tryptophan-Aspartic acid (WD) repeat containing protein, was the first nuclear pore complex protein to be associated with hereditary neurodegenerative disease and the only nuclear pore complex protein to be associated with hereditary adrenal disease. ALADIN's precise function at the nuclear pore complex is unknown. Most naturally occurring AAAS mutations result in mislocalization of the abnormal ALADIN protein (mainly into the cytoplasm), implying that correct NPC targeting is vital for its function. (PMID: 23825130)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALADIN antibody 15127-1-AP | Download protocol |
| IHC protocol for ALADIN antibody 15127-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |