- Featured Product
- KD/KO Validated
ALADIN Polyclonal antibody
ALADIN Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, monkey, mouse
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SH-SY5Y cells, NIH/3T3 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
15127-1-AP targets ALADIN in WB, IF, IHC, ELISA applications and shows reactivity with human, monkey, mouse samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, monkey, mouse |
| Cited Reactivity | human |
| Immunogen | ALADIN fusion protein Ag7211 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | achalasia, adrenocortical insufficiency, alacrimia (Allgrove, triple-A) |
| Synonyms | AAA, AAAS, AAASb, ADRACALA, ADRACALIN, ALADIN, GL003 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 56-60 kDa |
| GenBank Accession Number | BC000659 |
| Gene Symbol | AAAS |
| Gene ID (NCBI) | 8086 |
| RRID | AB_2219477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRG9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The AAAS gene product is the 60 kDa nuclear pore complex (NPC) protein alacrima-achalasia-adrenal insufficiency neurological disorder (ALADIN). AAAS mRNA and the ALADIN protein are ubiquitously expressed with predominance in the adrenal and central nervous system structures in human and rat. ALADIN, a Tryptophan-Aspartic acid (WD) repeat containing protein, was the first nuclear pore complex protein to be associated with hereditary neurodegenerative disease and the only nuclear pore complex protein to be associated with hereditary adrenal disease. ALADIN's precise function at the nuclear pore complex is unknown. Most naturally occurring AAAS mutations result in mislocalization of the abnormal ALADIN protein (mainly into the cytoplasm), implying that correct NPC targeting is vital for its function. (PMID: 23825130)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALADIN antibody 15127-1-AP | Download protocol |
| IHC protocol for ALADIN antibody 15127-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Biol Cell The Nucleoporin ALADIN Regulates Aurora A Localization to Ensure Robust Mitotic Spindle Formation. |