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AACS Polyclonal antibody

AACS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13815-1-AP

Print datasheet

Synonyms

Protein sur 5 homolog, EC:6.2.1.16, Acyl-CoA synthetase family member 1, ACSF1, ACSF 1

验证数据展示

  • WB analysis of mouse heart using 13815-1-AP

    WB analysis of mouse heart using 13815-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13815-1-AP (AACS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 13815-1-AP

    IF Staining of MCF-7 using 13815-1-AP

    Immunofluorescent analysis of MCF-7 cells using 13815-1-AP (AACS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human ovary cancer using 13815-1-AP

    IHC staining of human ovary cancer using 13815-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13815-1-AP (AACS antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse liver tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

13815-1-AP targets AACS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen AACS fusion protein Ag4899 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acetoacetyl-CoA synthetase
Synonyms Protein sur 5 homolog, EC:6.2.1.16, Acyl-CoA synthetase family member 1, ACSF1, ACSF 1
Calculated Molecular Weight 672 aa, 75 kDa
Observed Molecular Weight 67 kDa, 30 kDa
GenBank Accession NumberBC040490
Gene Symbol AACS
Gene ID (NCBI) 65985
RRIDAB_2877977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86V21
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for AACS antibody 13815-1-APDownload protocol
IHC protocol for AACS antibody 13815-1-APDownload protocol
IF protocol for AACS antibody 13815-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

iScience

Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice.

Authors - George D Dalton
View Article