验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse liver tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
13815-1-AP targets AACS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | AACS fusion protein Ag4899 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acetoacetyl-CoA synthetase |
| 别名 | Protein sur 5 homolog, EC:6.2.1.16, Acyl-CoA synthetase family member 1, ACSF1, ACSF 1 |
| 计算分子量 | 672 aa, 75 kDa |
| 观测分子量 | 67 kDa, 30 kDa |
| GenBank蛋白编号 | BC040490 |
| 基因名称 | AACS |
| Gene ID (NCBI) | 65985 |
| RRID | AB_2877977 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86V21 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AACS(acetoacetyl-CoA synthetase) is also named as ACSF1 and belonogs to the ATP-dependent AMP-binding enzyme family. In mammals, AACS is present in the cytosolic fractions of various tissues, especially lipogenic ones, and acetoacetate is known to be effectively incorporated into cholesterol and fatty acids. These results indicate that AACS may supply acetyl units to cytosolic compartment for cholesterol and/or fatty acid biosynthesis. It has 3 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AACS antibody 13815-1-AP | Download protocol |
| IHC protocol for AACS antibody 13815-1-AP | Download protocol |
| IF protocol for AACS antibody 13815-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Hepatocyte activity of the cholesterol sensor smoothened regulates cholesterol and bile acid homeostasis in mice. | ||
bioRxiv Ketogenesis supports hepatic polyunsaturated fatty acid homeostasis via fatty acid elongation | ||
bioRxiv An alternative route for β-hydroxybutyrate metabolism supports fatty acid synthesis in cancer cells
|
