AADACL1 Polyclonal antibody
AADACL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells |
Positive IP detected in | COLO 320 cells |
Positive IHC detected in | human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | COLO 320 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
14021-1-AP targets AADACL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | AADACL1 fusion protein Ag5136 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | arylacetamide deacetylase-like 1 |
Synonyms | EC:3.1.1.71, EC:3.1.1.-, Arylacetamide deacetylase-like 1, Acetylalkylglycerol acetylhydrolase, AADACL 1 |
Calculated Molecular Weight | 46 kDa |
Observed Molecular Weight | 40-50 kDa |
GenBank Accession Number | BC047588 |
Gene Symbol | AADACL1 |
Gene ID (NCBI) | 57552 |
RRID | AB_2034888 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q6PIU2 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for AADACL1 antibody 14021-1-AP | Download protocol |
IHC protocol for AADACL1 antibody 14021-1-AP | Download protocol |
IF protocol for AADACL1 antibody 14021-1-AP | Download protocol |
IP protocol for AADACL1 antibody 14021-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cardiovasc Diabetol Deficiency of neutral cholesterol ester hydrolase 1 (NCEH1) impairs endothelial function in diet-induced diabetic mice | ||
J Proteomics Integrative proteomics-metabolomics strategy reveals the mechanism of hepatotoxicity induced by Fructus Psoraleae. | ||
J Transl Med Multiplex immunohistochemistry defines two cholesterol metabolism patterns predicting immunotherapeutic outcomes in gastric cancer | ||
Sci Rep Lysophosphatidylglucoside/GPR55 signaling promotes foam cell formation in human M2c macrophages | ||
Naunyn Schmiedebergs Arch Pharmacol Molecular mechanism of geniposide against ANIT-induced intrahepatic cholestasis by integrative analysis of transcriptomics and metabolomics |