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AAMP Polyclonal antibody

AAMP Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21220-1-AP

Print datasheet

Synonyms

AAMP

验证数据展示

  • WB analysis using 21220-1-AP

    WB analysis using 21220-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 21220-1-AP

    IHC staining of human liver cancer using 21220-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21220-1-AP (AAMP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 21220-1-AP

    IHC staining of human liver cancer using 21220-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21220-1-AP (AAMP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse lung using 21220-1-AP

    WB analysis of mouse lung using 21220-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21220-1-AP (AAMP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 21220-1-AP

    IF Staining of MCF-7 using 21220-1-AP

    Immunofluorescent analysis of MCF-7 cells using 21220-1-AP (AAMP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHUVEC cells, human heart tissue, mouse kidney tissue, mouse lung tissue, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21220-1-AP is AAMP Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, WB,ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityrat
Immunogen AAMP fusion protein Ag15655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name angio-associated, migratory cell protein
Synonyms AAMP
Calculated Molecular Weight 434 aa, 47 kDa
Observed Molecular Weight 52-58 kDa
GenBank Accession NumberBC039866
Gene Symbol AAMP
Gene ID (NCBI) 14
RRIDAB_10733642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13685
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AAMP antibody 21220-1-APDownload protocol
IHC protocol for AAMP antibody 21220-1-APDownload protocol
IF protocol for AAMP antibody 21220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

Exp Ther Med

MicroRNA-125b inhibits the proliferation of vascular smooth muscle cells induced by platelet-derived growth factor BB.

Authors - Xiaogao Wang
View Article