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AARS2 Polyclonal antibody

AARS2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 22696-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, IP, ELISA

AARSL, Alanine tRNA ligase, Alanine--tRNA ligase, mitochondrial, Alanyl tRNA synthetase like, Alanyl-tRNA synthetase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22696-1-AP targets AARS2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 AARS2 fusion protein Ag18539 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alanyl-tRNA synthetase 2, mitochondrial (putative)
别名 AARSL, Alanine tRNA ligase, Alanine--tRNA ligase, mitochondrial, Alanyl tRNA synthetase like, Alanyl-tRNA synthetase
计算分子量 985 aa, 107 kDa
观测分子量 105-115 kDa
GenBank蛋白编号BC131728
基因名称 AARS2
Gene ID (NCBI) 57505
RRIDAB_11182926
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JTZ9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AARS2(Alanine--tRNA ligase, mitochondrial) is also named as KIAA1270, AARSL, bA444E17.1, AlaRS, AARSL, COXPD8, MTALARS, MT-ALARS and belongs to the class-II aminoacyl-tRNA synthetase family. It catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). It also edits incorrectly charged tRNA(Ala) via its editing domain. The full length 107 kDa protein has a transit peptide with 23 amino acids.

实验方案

Product Specific Protocols
WB protocol for AARS2 antibody 22696-1-APDownload protocol
IHC protocol for AARS2 antibody 22696-1-APDownload protocol
IP protocol for AARS2 antibody 22696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Res

Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation

Authors - Yunzi Mao
  • KO Validated
ratWB

Cell Stem Cell

A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.

Authors - Jianyong Du
humanWB

J Hematol Oncol

Integrated proteogenomic characterization of urothelial carcinoma of the bladder.

Authors - Ning Xu
humanWB

Cancer Med

AARS2 as a novel biomarker for prognosis and its molecular characterization in pan-cancer

Authors - Long Liu
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