验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells |
Positive IP detected in | K-562 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 4 publications below |
产品信息
22696-1-AP targets AARS2 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human, mouse, rat |
免疫原 | AARS2 fusion protein Ag18539 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | alanyl-tRNA synthetase 2, mitochondrial (putative) |
别名 | AARSL, Alanine tRNA ligase, Alanine--tRNA ligase, mitochondrial, Alanyl tRNA synthetase like, Alanyl-tRNA synthetase |
计算分子量 | 985 aa, 107 kDa |
观测分子量 | 105-115 kDa |
GenBank蛋白编号 | BC131728 |
基因名称 | AARS2 |
Gene ID (NCBI) | 57505 |
RRID | AB_11182926 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q5JTZ9 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AARS2(Alanine--tRNA ligase, mitochondrial) is also named as KIAA1270, AARSL, bA444E17.1, AlaRS, AARSL, COXPD8, MTALARS, MT-ALARS and belongs to the class-II aminoacyl-tRNA synthetase family. It catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). It also edits incorrectly charged tRNA(Ala) via its editing domain. The full length 107 kDa protein has a transit peptide with 23 amino acids.
实验方案
Product Specific Protocols | |
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WB protocol for AARS2 antibody 22696-1-AP | Download protocol |
IHC protocol for AARS2 antibody 22696-1-AP | Download protocol |
IP protocol for AARS2 antibody 22696-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation
| ||
Cell Stem Cell A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration. | ||
J Hematol Oncol Integrated proteogenomic characterization of urothelial carcinoma of the bladder. | ||
Cancer Med AARS2 as a novel biomarker for prognosis and its molecular characterization in pan-cancer |