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验证数据展示

  • WB analysis using 14900-1-AP

    WB analysis using 14900-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14900-1-AP (AARSD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 14900-1-AP

    IHC staining of mouse kidney using 14900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 14900-1-AP

    IHC staining of mouse kidney using 14900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AARSD1 Polyclonal antibody

AARSD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14900-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AARSD1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue
Positive IHC detected inmouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14900-1-AP targets AARSD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AARSD1 fusion protein Ag6692 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alanyl-tRNA synthetase domain containing 1
别名 AARSD1
计算分子量 45 kDa
观测分子量 45 kDa
GenBank蛋白编号BC004172
基因名称 AARSD1
Gene ID (NCBI) 80755
RRIDAB_2257630
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BTE6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AARSD1(Alanyl-tRNA synthetase domain-containing protein 1) is also named as alanyl-tRNA editing protein Aarsd1 and belongs to the class-II aminoacyl-tRNA synthetase family. It plays a role in prevention of synthesis of proteins containing errors (mistranslation)(PMID:18172502). It has 3 isoforms produced by alternative splicing with the molecular weight of 45 kDa, 59 kDa and 66 kDa.

实验方案

Product Specific Protocols
WB protocol for AARSD1 antibody 14900-1-APDownload protocol
IHC protocol for AARSD1 antibody 14900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols