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AARSD1 Polyclonal antibody

AARSD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14900-1-AP

Print datasheet

Synonyms

AARSD1

验证数据展示

  • WB analysis using 14900-1-AP

    WB analysis using 14900-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14900-1-AP (AARSD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 14900-1-AP

    IHC staining of mouse kidney using 14900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 14900-1-AP

    IHC staining of mouse kidney using 14900-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue
Positive IHC detected inmouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14900-1-AP targets AARSD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AARSD1 fusion protein Ag6692 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name alanyl-tRNA synthetase domain containing 1
Synonyms AARSD1
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC004172
Gene Symbol AARSD1
Gene ID (NCBI) 80755
RRIDAB_2257630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BTE6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AARSD1(Alanyl-tRNA synthetase domain-containing protein 1) is also named as alanyl-tRNA editing protein Aarsd1 and belongs to the class-II aminoacyl-tRNA synthetase family. It plays a role in prevention of synthesis of proteins containing errors (mistranslation)(PMID:18172502). It has 3 isoforms produced by alternative splicing with the molecular weight of 45 kDa, 59 kDa and 66 kDa.

实验方案

Product Specific Protocols
WB protocol for AARSD1 antibody 14900-1-APDownload protocol
IHC protocol for AARSD1 antibody 14900-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols