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验证数据展示

  • WB analysis using 10282-1-AP

    WB analysis using 10282-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10282-1-AP (AATF antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10282-1-AP

    IF Staining of HeLa using 10282-1-AP

    Immunofluorescent analysis of Hela cells, using AATF antibody 10282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis of HepG2 using 10282-1-AP

    WB analysis of HepG2 using 10282-1-AP

    WB result of AATF antibody (10282-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AATF transfected HepG2 cells.

  • Featured Product
  • KD/KO验证

AATF Polyclonal antibody

AATF Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 10282-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Protein AATF, HSPC277, DED, CHE1, CHE 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, mouse brain tissue, HepG2 cells, HeLa cells, NIH/3T3 cells
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10282-1-AP targets AATF in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AATF fusion protein Ag0195 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apoptosis antagonizing transcription factor
别名 Protein AATF, HSPC277, DED, CHE1, CHE 1
计算分子量 63 kDa
观测分子量80-95 kDa
GenBank蛋白编号BC000591
基因名称 AATF
Gene ID (NCBI) 26574
RRIDAB_2219887
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NY61
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apoptosis antagonizing transcription factor (AATF) is a nuclear phosphoprotein of 523 amino acids and contains an extremely acidic domain and a putative leucine zipper characteristic of transcription factors. AATF was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. Overexpression of this gene interfered with MAP3K12 induced apoptosis. AATF also binds Rb and the core of pol II, and may be part of transcription regulatory complex.

实验方案

Product Specific Protocols
WB protocol for AATF antibody 10282-1-APDownload protocol
IF protocol for AATF antibody 10282-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols