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验证数据展示

  • WB analysis of mouse thymus using 20681-1-AP

    WB analysis of mouse thymus using 20681-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20681-1-AP (ABCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 20681-1-AP

    IF Staining of HepG2 using 20681-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 20681-1-AP (ABCA2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse brain using 20681-1-AP

    IHC staining of mouse brain using 20681-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20681-1-AP (ABCA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ABCA2 Polyclonal antibody

ABCA2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20681-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ABC transporter ABCA2, ABC2, ABCA2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse thymus tissue, HepG2 cells, L02 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20681-1-AP targets ABCA2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family A (ABC1), member 2
别名 ABC transporter ABCA2, ABC2, ABCA2
计算分子量 270 kDa
观测分子量 270 kDa
GenBank蛋白编号NM_001606
基因名称 ABCA2
Gene ID (NCBI) 20
RRIDAB_10859780
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCA2, also named as ABC2 and KIAA1062, belongs to the ABC transporter ABCA2, a member of the ATP-binding cassette (ABC) transporter family, is primarily expressed in brain and is involved in brain sterol homeostasis and associated with early onset Alzheimer's disease. ABCA2 is mainly localized in endosomes and lysosomes of oligodendrocytes and Schwann cells. It has also be reported as a marker of neural progenitor in the adult rodent brain. (PMID: 16539677)

实验方案

Product Specific Protocols
WB protocol for ABCA2 antibody 20681-1-APDownload protocol
IHC protocol for ABCA2 antibody 20681-1-APDownload protocol
IF protocol for ABCA2 antibody 20681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols