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验证数据展示

  • WB analysis using 27450-1-AP

    WB analysis using 27450-1-AP

    unboiled rat lung tissue were subjected to SDS PAGE followed by western blot with 27450-1-AP (ABCA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ABCA3 Polyclonal antibody

ABCA3 Polyclonal Antibody for WB, ELISA
Cat No. 27450-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, ELISA

别名

ATP binding cassette 3, ABC-C transporter, ABCA 3, ABC3, ABC C transporter

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inunboiled rat lung tissue
Planning an IHC experiment? We recommend our IHCeasy ABCA3 Ready-To-Use IHC Kit. ABCA3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27450-1-AP targets ABCA3 in WB, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, rat
免疫原 ABCA3 fusion protein Ag24749 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family A (ABC1), member 3
别名 ATP binding cassette 3, ABC-C transporter, ABCA 3, ABC3, ABC C transporter
观测分子量 140-160 kDa
GenBank蛋白编号BC140895
基因名称 ABCA3
Gene ID (NCBI) 21
RRIDAB_3669603
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99758
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCA3 belongs to the ATP-binding cassette (ABC) transporter superfamily. ABCA3, which is expressed in alveolar type II pneumocytes and localizes predominantly to the limiting membrane of lamellar bodies, is critical for synthesis of surfactant(PMID: 17267394). Mutation of the ABCA3 gene causes fatal surfactant deficiency in newborns(PMID: 15044640).

实验方案

Product Specific Protocols
WB protocol for ABCA3 antibody 27450-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols