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ABCA8 Polyclonal antibody

ABCA8 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24351-1-AP

Print datasheet

Synonyms

ABCA8, KIAA0822

验证数据展示

  • WB analysis of mouse brain using 24351-1-AP

    WB analysis of mouse brain using 24351-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24351-1-AP (ABCA8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 24351-1-AP

    IHC staining of human liver using 24351-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24351-1-AP (ABCA8 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 24351-1-AP

    IHC staining of human liver using 24351-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24351-1-AP (ABCA8 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24351-1-AP targets ABCA8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ABCA8 fusion protein Ag18991 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family A (ABC1), member 8
Synonyms ABCA8, KIAA0822
Calculated Molecular Weight 1581 aa, 179 kDa
Observed Molecular Weight 160-180 kDa
GenBank Accession Numberbc130280
Gene Symbol ABCA8
Gene ID (NCBI) 10351
RRIDAB_2879504
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94911
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABCA8 antibody 24351-1-APDownload protocol
IHC protocol for ABCA8 antibody 24351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Cancer Cell

Tumor cell-intrinsic epigenetic dysregulation shapes cancer-associated fibroblasts heterogeneity to metabolically support pancreatic cancer

Authors - Ningning Niu
View Article