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  • Featured Product
  • KD/KO Validated

ABCB10 Polyclonal antibody

ABCB10 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14628-1-AP

Print datasheet

Synonyms

ABC transporter 10 protein, ABCB10, ABC-me, EST20237, M ABC2, MTABC2

验证数据展示

  • IP experiment of K-562 using 14628-1-AP

    IP experiment of K-562 using 14628-1-AP

    IP result of anti-ABCB10 (IP:14628-1-AP, 4ug; Detection:14628-1-AP 1:300) with K-562 cells lysate 3200ug.

  • WB analysis using 14628-1-AP

    WB analysis using 14628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14628-1-AP (ABCB10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells, K-562 cellls
Positive IP detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14628-1-AP targets ABCB10 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ABCB10 fusion protein Ag6203 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family B (MDR/TAP), member 10
Synonyms ABC transporter 10 protein, ABCB10, ABC-me, EST20237, M ABC2, MTABC2
Calculated Molecular Weight 79 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC064930
Gene Symbol ABCB10
Gene ID (NCBI) 23456
RRIDAB_2220668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRK6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABCB10 antibody 14628-1-APDownload protocol
IP protocol for ABCB10 antibody 14628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

ABCB10 depletion reduces unfolded protein response in mitochondria.

Authors - Masato Yano
  • KD Validated
View Article
humanWB

PeerJ

TMEM65 is a mitochondrial inner-membrane protein.

Authors - Naotaka Nishimura
View Article
mouseFC

Exp Hematol

Abcb10 regulates murine hematopoietic stem cell potential and erythroid differentiation

Authors - Ayano Yahagi
View Article

Mol Cell

Unraveling ETC complex I function in ferroptosis reveals a potential ferroptosis-inducing therapeutic strategy for LKB1-deficient cancers

Authors - Chao Mao
View Article