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验证数据展示

  • WB analysis of mouse brain using 14996-1-AP

    WB analysis of mouse brain using 14996-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14996-1-AP (ABCB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 14996-1-AP

    IHC staining of human breast cancer using 14996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 14996-1-AP

    IHC staining of human breast cancer using 14996-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ABCB6 Polyclonal antibody

ABCB6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14996-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ABC, ABC14, ABCB6, EST45597, Mt ABC transporter 3, MTABC3, P glycoprotein related protein, PRP, umat

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14996-1-AP targets ABCB6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ABCB6 fusion protein Ag6982 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family B (MDR/TAP), member 6
别名 ABC, ABC14, ABCB6, EST45597, Mt ABC transporter 3, MTABC3, P glycoprotein related protein, PRP, umat
计算分子量 94 kDa
观测分子量 170 kDa
GenBank蛋白编号BC000559
基因名称 ABCB6
Gene ID (NCBI) 10058
RRIDAB_2220828
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NP58
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABCB6 antibody 14996-1-APDownload protocol
IHC protocol for ABCB6 antibody 14996-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols