验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, mouse thymus tissue |
| Positive IP detected in | mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
11158-1-AP targets ABCB7 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | ABCB7 fusion protein Ag1544 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ATP-binding cassette, sub-family B (MDR/TAP), member 7 |
| 别名 | ABC transporter 7 protein, ABC7, ABCB7, ASAT, Atm1p, EST140535 |
| 计算分子量 | 753 aa, 83 kDa |
| 观测分子量 | 83 kDa |
| GenBank蛋白编号 | BC006323 |
| 基因名称 | ABCB7 |
| Gene ID (NCBI) | 22 |
| RRID | AB_2220945 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75027 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABCB7 belongs to the ATP-binding cassette (ABC) transporter superfamily. ABCB7 exports glutathione-coordinated iron-sulfur clusters from the mitochondria to the cytosol in an ATP-dependent manner allowing the assembly of the cytosolic iron-sulfur-containing proteins and participates in iron homeostasis(PMID: 33157103, 17192393). ABCB7 structure and mutation causing X- linked sideroblastic anemia and ataxia (XLSA/A) with disruption of cytosolic iron-sulfur protein maturation (PMID: 10196363,11050011).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ABCB7 antibody 11158-1-AP | Download protocol |
| IP protocol for ABCB7 antibody 11158-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Augmenter of liver regeneration regulates cellular iron homeostasis by modulating mitochondrial transport of ATP-binding cassette B8. | ||
Arch Biochem Biophys Knockdown of ABCB7 inhibits esophageal cancer progression by inhibiting the TGF-β/Smad signaling
| ||
Mol Cell Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis |
