• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ABCC10 Polyclonal antibody

ABCC10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30697-1-AP

Print datasheet

Synonyms

ABCC10, MRP7, SIMRP7

验证数据展示

  • WB analysis using 30697-1-AP

    WB analysis using 30697-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30697-1-AP (ABCC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 30697-1-AP

    IHC staining of mouse liver using 30697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30697-1-AP (ABCC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 30697-1-AP

    IHC staining of mouse liver using 30697-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30697-1-AP (ABCC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30697-1-AP targets ABCC10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse
Immunogen ABCC10 fusion protein Ag33719 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family C (CFTR/MRP), member 10
Synonyms ABCC10, MRP7, SIMRP7
Calculated Molecular Weight161kd
Observed Molecular Weight~190 kDa
GenBank Accession NumberNM_001198934.1
Gene Symbol ABCC10
Gene ID (NCBI) 89845
RRIDAB_3086392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T3U5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ATP binding cassette sub-family C member 10 (ABCC10), also named MRP7, is a cellular transmembrane protein transporter and belongs to the ATP-binding cassette (ABC) family. MRP7 is a lipophilic anion pump responsible for the transport of glucuronide E(2)17betaG and can confer resistance to chemotherapy drugs (PMID: 16868766). Since it is glycosylated, the apparent molecular weight of ABCC10 could be variable, ranging from 161 kDa to 191 kDa (PMID: 23087055; PMID: 24103790; PMID: 31338999; PMID: 34631561).

实验方案

Product Specific Protocols
WB protocol for ABCC10 antibody 30697-1-APDownload protocol
IHC protocol for ABCC10 antibody 30697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols