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验证数据展示

  • WB analysis using 29261-1-AP

    WB analysis using 29261-1-AP

    37°C incubated A549 cells total extract lysate and 37°C incubated HepG2 cells total extract lysate were subjected to SDS PAGE followed by western blot with 29261-1-AP (MRP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 29261-1-AP

    IHC staining of human liver using 29261-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29261-1-AP (ABCC2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 29261-1-AP

    IHC staining of human liver using 29261-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 29261-1-AP (ABCC2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MRP2 Polyclonal antibody

MRP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29261-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

ABC30, ABCC2, CMOAT, CMOAT1, cMRP, DJS, KIAA1010, MRP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in37°C incubated A549 cells, 37°C incubated HepG2 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29261-1-AP targets MRP2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 MRP2 fusion protein Ag30944 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family C (CFTR/MRP), member 2
别名 ABC30, ABCC2, CMOAT, CMOAT1, cMRP, DJS, KIAA1010, MRP2
计算分子量 1545 aa, 174 kDa
观测分子量 190-250 kDa
GenBank蛋白编号BC136419
基因名称 ABCC2
Gene ID (NCBI) 1244
RRIDAB_3086108
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92887
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Multi-drug resistance protein 2 (MRP2), also known as ABCC2, is an ATP binding cassette (ABC) transporter responsible for biliary excretion of xenobiotics, endobiotics, and their metabolites. Deficiency in ABCC2 results in the clinical disorder Dubin-Johnson syndrome. MRP2 is found to be expressed in a variety of human cancers, and is associated with resistance of tumor cells to various anticancer drugs including cisplatin. The predicted molecular weight of MRP2 is 174 kDa, while mature MRP2 usually has a slower migration around 190-250 kDa due to the glycosylation. (16434545, 18834541, 23045960)

实验方案

Product Specific Protocols
WB protocol for MRP2 antibody 29261-1-APDownload protocol
IHC protocol for MRP2 antibody 29261-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols