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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84204-5-RR (same clone as 84204-5-PBS)

    WB analysis using 84204-5-RR (same clone as 84204-5-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 84204-5-RR (ABCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84204-5-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84204-5-RR (same clone as 84204-5-PBS)

    IHC staining of human ovary cancer using 84204-5-RR (same clone as 84204-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84204-5-RR (ABCC5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84204-5-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 84204-5-RR (same clone as 84204-5-PBS)

    IHC staining of human ovary cancer using 84204-5-RR (same clone as 84204-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84204-5-RR (ABCC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84204-5-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 84204-5-RR (same clone as 84204-5-PBS)

    IF Staining of PC-3 using 84204-5-RR (same clone as 84204-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using ABCC5 antibody (84204-5-RR, Clone: 241380G1 ) at dilution of 1:0 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84204-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84204-5-RR

    Affinity and Kinetic Characterization of 84204-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84204-5-RR against Human ABCC5 were performed. The affinity constant is 2.18 nM.

ABCC5 Recombinant antibody, PBS Only

ABCC5 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84204-5-PBS

产品说明书

CloneNo. 241380G1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MRP5, MOAT C, EC:7.6.2.-, ATP-binding cassette sub-family C member 5, ABCC 5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84204-5-PBS targets ABCC5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 ABCC5 fusion protein Ag31680 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ATP-binding cassette, sub-family C (CFTR/MRP), member 5
别名 MRP5, MOAT C, EC:7.6.2.-, ATP-binding cassette sub-family C member 5, ABCC 5
计算分子量 161 kDa
观测分子量161-200 kDa
GenBank蛋白编号NM_005688
基因名称 ABCC5
Gene ID (NCBI) 10057
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO15440
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ABCC5, also named MOAT-C, pABC11, SMRP, and MRP5, belongs to the ABC transporter superfamily, ABCC family, and Conjugate transporter (TC 3.A.1.208) subfamily. ABCC5 acts as a multispecific organic anion pump that can transport nucleotide analogs. ABCC5 functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that ABCC5 provides resistance to thiopurine anticancer drugs, 6-mercaptopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl) adenine. ABCC5 may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing of this gene has been detected; however, the complete sequence and translation initiation site are unclear. Since it is glycosylated, the apparent molecular weight of ABCC5 could be variable, ranging from 161 kDa to 200 kDa (PMID: 10893247; PMID: 15897250; PMID: 31338999).