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- KD/KO Validated
ABCD1 Polyclonal antibody
ABCD1 Polyclonal Antibody for WB, IP, IF/ICC, ELISA, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IF/ICC, ELISA, FC (Intra)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HeLa cells |
Positive IP detected in | HeLa cells |
Positive IF detected in | HeLa cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 2 publications below |
产品信息
18138-1-AP targets ABCD1 in WB, IP, IF, ELISA, FC (Intra) applications and shows reactivity with human samples.
Tested Applications | WB, IP, IF/ICC, ELISA, FC (Intra) Application Description |
Cited Applications | WB |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | ABCD1 fusion protein Ag12757 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ATP-binding cassette, sub-family D (ALD), member 1 |
Synonyms | ABCD1, ALD, ATP binding cassette sub family D member 1, ATP-binding cassette sub-family D member 1, EC:3.1.2.-, ABCD 1 |
Calculated Molecular Weight | 745 aa, 83 kDa |
Observed Molecular Weight | 75 kDa |
GenBank Accession Number | BC025358 |
Gene Symbol | ABCD1 |
Gene ID (NCBI) | 215 |
RRID | AB_2273333 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P33897 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ABCD1 antibody 18138-1-AP | Download protocol |
IF protocol for ABCD1 antibody 18138-1-AP | Download protocol |
IP protocol for ABCD1 antibody 18138-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.
| ||
Signal Transduct Target Ther Structural insights into substrate recognition and translocation of human peroxisomal ABC transporter ALDP |