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  • Featured Product
  • KD/KO Validated

ABCE1 Polyclonal antibody

ABCE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28548-1-AP

Print datasheet

Synonyms

ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I

验证数据展示

  • WB analysis using 28548-1-AP

    WB analysis using 28548-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28548-1-AP (ABCE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 28548-1-AP

    IHC staining of human breast cancer using 28548-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28548-1-AP (ABCE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCE1 antibody included
  • IHC staining of human ovary tumor using 28548-1-AP

    IHC staining of human ovary tumor using 28548-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 28548-1-AP (ABCE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCE1 antibody included
  • IHC staining of human gliomas using 28548-1-AP

    IHC staining of human gliomas using 28548-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 28548-1-AP (ABCE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ABCE1 antibody included

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, NCI-H1299 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ABCE1 Ready-To-Use IHC Kit. ABCE1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28548-1-AP targets ABCE1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen ABCE1 fusion protein Ag29724 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family E (OABP), member 1
Synonyms ABC38, ABCE1, HuHP68, OABP, Ribonuclease 4 inhibitor, RLI, RNase L inhibitor, RNASEL1, RNASELI, RNS4I
Calculated Molecular Weight 599 aa, 67 kDa
Observed Molecular Weight68 kDa
GenBank Accession NumberBC016283
Gene Symbol ABCE1
Gene ID (NCBI) 6059
RRIDAB_2881169
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61221
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABCE1 antibody 28548-1-APDownload protocol
IHC protocol for ABCE1 antibody 28548-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

Int Immunopharmacol

Restoration of miR-299-3p promotes macrophage phagocytosis and suppresses malignant phenotypes in breast cancer carcinogenesis via dual-targeting CD47 and ABCE1

Authors - Shoufang Tong
  • KD Validated
View Article
humanWB

J Clin Invest

CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes

Authors - Nunziata Maio
View Article