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验证数据展示

  • WB analysis using 15984-1-AP

    WB analysis using 15984-1-AP

    Mouse heart lysates were subjected to SDS PAGE followed by western blot with 15984-1-AP (ABCF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 15984-1-AP

    IHC staining of human ovary cancer using 15984-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15984-1-AP (ABCF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ABCF3 Polyclonal antibody

ABCF3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15984-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ATP-binding cassette sub-family F member 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15984-1-AP targets ABCF3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ABCF3 fusion protein Ag8787 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ATP-binding cassette, sub-family F (GCN20), member 3
别名 ATP-binding cassette sub-family F member 3
计算分子量 709 aa, 80 kDa
观测分子量 80 kDa
GenBank蛋白编号BC009253
基因名称 ABCF3
Gene ID (NCBI) 55324
RRIDAB_2878205
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NUQ8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABCF3 antibody 15984-1-APDownload protocol
IHC protocol for ABCF3 antibody 15984-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols