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ABCG4 Polyclonal antibody

ABCG4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14269-1-AP

Print datasheet

Synonyms

ABCG4, WHITE2

验证数据展示

  • WB analysis of U-937 using 14269-1-AP

    WB analysis of U-937 using 14269-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 14269-1-AP (ABCG4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 14269-1-AP

    IHC staining of human liver cancer using 14269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14269-1-AP (ABCG4 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 14269-1-AP

    IHC staining of human liver cancer using 14269-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14269-1-AP (ABCG4 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inU-937 cells, mouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14269-1-AP targets ABCG4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ABCG4 fusion protein Ag5587 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP-binding cassette, sub-family G (WHITE), member 4
Synonyms ABCG4, WHITE2
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 60 kDa, 90-100 kDa
GenBank Accession NumberBC041091
Gene Symbol ABCG4
Gene ID (NCBI) 64137
RRIDAB_2220436
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H172
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABCG4 antibody 14269-1-APDownload protocol
IHC protocol for ABCG4 antibody 14269-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IHC

PLoS One

High ABCG4 Expression Is Associated with Poor Prognosis in Non-Small-Cell Lung Cancer Patients Treated with Cisplatin-Based Chemotherapy.

Authors - Guang Yang
View Article