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ABHD10 Polyclonal antibody

ABHD10 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22558-1-AP

Print datasheet

Synonyms

验证数据展示

  • WB analysis of HeLa using 22558-1-AP

    WB analysis of HeLa using 22558-1-AP

    WB result of ABHD10 antibody (22558-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ABHD10 transfected HeLa cells.

  • WB analysis using 22558-1-AP

    WB analysis using 22558-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22558-1-AP (ABHD10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inY79 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22558-1-AP targets ABHD10 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen ABHD10 fusion protein Ag18413 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name abhydrolase domain containing 10
Synonyms
Calculated Molecular Weight 306 aa, 34 kDa
Observed Molecular Weight28 kDa
GenBank Accession NumberBC014516
Gene Symbol ABHD10
Gene ID (NCBI) 55347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NUJ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABHD10 is a mitochondrial protein that is a member of the APT family. It has 3 isoforms and regulates the S-palmitoylation status of the nucleophilic active site residue of peroxiredoxin-5 (PRDX5) (PMID: 31740833).

实验方案

Product Specific Protocols
WB protocol for ABHD10 antibody 22558-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols