• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ABHD12B Polyclonal antibody

ABHD12B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20623-1-AP

Print datasheet

Synonyms

ABHD12B, BEM46L3, c14_5314, C14orf29

验证数据展示

  • WB analysis of mouse skin using 20623-1-AP

    WB analysis of mouse skin using 20623-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 20623-1-AP (ABHD12B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 20623-1-AP

    IHC staining of human skin using 20623-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20623-1-AP (ABHD12B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 20623-1-AP

    IHC staining of human skin using 20623-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 20623-1-AP (ABHD12B antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skin tissue, mouse kidney tissue
Positive IHC detected inhuman skin tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20623-1-AP is ABHD12B Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ABHD12B fusion protein Ag14426 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name abhydrolase domain containing 12B
Synonyms ABHD12B, BEM46L3, c14_5314, C14orf29
Calculated Molecular Weight 362 aa, 41 kDa
Observed Molecular Weight 41 kDa, 29 kDa
GenBank Accession NumberBC034603
Gene Symbol ABHD12B
Gene ID (NCBI) 145447
RRIDAB_10693623
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z5M8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABHD12B antibody 20623-1-APDownload protocol
IHC protocol for ABHD12B antibody 20623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols