• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of L02 using 20952-1-AP

    WB analysis of L02 using 20952-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20952-1-AP (ABHD14B antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 20952-1-AP

    IHC staining of human prostate hyperplasia using 20952-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ABHD14B antibody included
  • IHC staining of human prostate hyperplasia using 20952-1-AP

    IHC staining of human prostate hyperplasia using 20952-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia using 20952-1-AP (ABHD14B antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ABHD14B antibody included
  • IF Staining of HepG2 using 20952-1-AP

    IF Staining of HepG2 using 20952-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ABHD14B antibody (20952-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ABHD14B Polyclonal antibody

ABHD14B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20952-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Putative protein-lysine deacylase ABHD14B, EC:2.3.1.-, CIB, CCG1-interacting factor B, Alpha/beta hydrolase domain-containing protein 14B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inL02 cells, mouse small intestine tissue
Positive IHC detected inhuman prostate hyperplasia tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ABHD14B Ready-To-Use IHC Kit. ABHD14B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20952-1-AP targets ABHD14B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ABHD14B fusion protein Ag15097 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 abhydrolase domain containing 14B
别名 Putative protein-lysine deacylase ABHD14B, EC:2.3.1.-, CIB, CCG1-interacting factor B, Alpha/beta hydrolase domain-containing protein 14B
计算分子量 210 aa, 22 kDa
观测分子量 22-25 kDa
GenBank蛋白编号BC007234
基因名称 ABHD14B
Gene ID (NCBI) 84836
RRIDAB_10733758
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96IU4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABHD14B antibody 20952-1-APDownload protocol
IHC protocol for ABHD14B antibody 20952-1-APDownload protocol
IF protocol for ABHD14B antibody 20952-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols