验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, A549 cells, HCT 116 cells, HeLa cells, HepG2 cells, LNCaP cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon tissue, human kidney tissue, human liver tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12201-1-AP targets ABHD5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | ABHD5 fusion protein Ag2840 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | abhydrolase domain containing 5 |
| 别名 | IECN2, EC:2.3.1.51, CGI58, CDS, Abhydrolase domain-containing protein 5 |
| 计算分子量 | 349 aa, 39 kDa |
| 观测分子量 | 39 kDa |
| GenBank蛋白编号 | BC021958 |
| 基因名称 | ABHD5 |
| Gene ID (NCBI) | 51099 |
| RRID | AB_2220710 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WTS1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ABHD5 antibody 12201-1-AP | Download protocol |
| IHC protocol for ABHD5 antibody 12201-1-AP | Download protocol |
| IF protocol for ABHD5 antibody 12201-1-AP | Download protocol |
| IP protocol for ABHD5 antibody 12201-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun PNPLA3 is a triglyceride lipase that mobilizes polyunsaturated fatty acids to facilitate hepatic secretion of large-sized very low-density lipoprotein | ||
Nat Commun O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. | ||
Cell Death Differ Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation. | ||
Proc Natl Acad Sci U S A Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover. | ||
Proc Natl Acad Sci U S A Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis. | ||
J Cell Biol ORP5 localizes to ER-lipid droplet contacts and regulates the level of PI(4)P on lipid droplets. |
