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验证数据展示

  • IHC staining of human brain using 20494-1-AP

    IHC staining of human brain using 20494-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 20494-1-AP

    IHC staining of human brain using 20494-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 20494-1-AP

    WB analysis using 20494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20494-1-AP (ABHD6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

ABHD6 Polyclonal antibody

ABHD6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20494-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

EC:3.1.1.23, Abhydrolase domain-containing protein 6, ABHD 6, 2-arachidonoylglycerol hydrolase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat spleen tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20494-1-AP targets ABHD6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 ABHD6 fusion protein Ag13968 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 abhydrolase domain containing 6
别名 EC:3.1.1.23, Abhydrolase domain-containing protein 6, ABHD 6, 2-arachidonoylglycerol hydrolase
计算分子量 337 aa, 38 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC001698
基因名称 ABHD6
Gene ID (NCBI) 57406
RRIDAB_2878694
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BV23
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

α/β-hydrolase domain 6 (ABHD6) is a lipase linked to physiological functions affecting energy metabolism. ABHD6, which was first identified in the brain ( PMID: 18096503) , is a ubiquitously expressed lipase.

实验方案

Product Specific Protocols
WB protocol for ABHD6 antibody 20494-1-APDownload protocol
IHC protocol for ABHD6 antibody 20494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Neuron

An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.

Authors - Jochen Schwenk
View Article
mouseWB

Pharmacol Res

Wwl70-induced ABHD6 inhibition attenuates memory deficits and pathological phenotypes in APPswe/PS1dE9 mice

Authors - Zhiwei Xue
View Article
humanWB

Mol Carcinog

ABHD6 suppresses colorectal cancer progression via AKT signaling pathway

Authors - Xiaoyu Xiong
  • KD Validated
View Article