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  • Featured Product
  • KD/KO Validated

ABHD6 Polyclonal antibody

ABHD6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 20494-1-AP

Print datasheet

Synonyms

EC:3.1.1.23, Abhydrolase domain-containing protein 6, ABHD 6, 2-arachidonoylglycerol hydrolase

验证数据展示

  • WB analysis of RAW 264.7 using 20494-1-AP

    WB analysis of RAW 264.7 using 20494-1-AP

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 20494-1-AP (ABHD6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 20494-1-AP

    IHC staining of human brain using 20494-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 20494-1-AP

    IHC staining of human brain using 20494-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 20494-1-AP (ABHD6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inRAW 264.7 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20494-1-AP targets ABHD6 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ABHD6 fusion protein Ag13968 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name abhydrolase domain containing 6
Synonyms EC:3.1.1.23, Abhydrolase domain-containing protein 6, ABHD 6, 2-arachidonoylglycerol hydrolase
Calculated Molecular Weight 337 aa, 38 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC001698
Gene Symbol ABHD6
Gene ID (NCBI) 57406
RRIDAB_2878694
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BV23
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABHD6 antibody 20494-1-APDownload protocol
IHC protocol for ABHD6 antibody 20494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Neuron

An ER Assembly Line of AMPA-Receptors Controls Excitatory Neurotransmission and Its Plasticity.

Authors - Jochen Schwenk
View Article
mouseWB

Pharmacol Res

Wwl70-induced ABHD6 inhibition attenuates memory deficits and pathological phenotypes in APPswe/PS1dE9 mice

Authors - Zhiwei Xue
View Article
humanWB

Mol Carcinog

ABHD6 suppresses colorectal cancer progression via AKT signaling pathway

Authors - Xiaoyu Xiong
  • KD Validated
View Article