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验证数据展示

  • WB analysis using 16227-1-AP

    WB analysis using 16227-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16227-1-AP (ABI3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 16227-1-AP

    IHC staining of mouse heart using 16227-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16227-1-AP (ABI3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 16227-1-AP

    IHC staining of mouse heart using 16227-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16227-1-AP (ABI3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 16227-1-AP

    IF Staining of U2OS using 16227-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using ABI3 antibody (16227-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

ABI3 Polyclonal antibody

ABI3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16227-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

SSH3BP3, New molecule including SH3, Nesh, ABI gene family member 3, ABI family, member 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, SK-N-SH cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16227-1-AP targets ABI3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ABI3 fusion protein Ag8164 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ABI family, member 3
别名 SSH3BP3, New molecule including SH3, Nesh, ABI gene family member 3, ABI family, member 3
计算分子量 366 aa, 39 kDa
观测分子量50 kDa
GenBank蛋白编号BC007780
基因名称 ABI3
Gene ID (NCBI) 51225
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2A4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABI family member 3 belongs to the ABL Interactors (ABI) family of proteins, which also includes ABI1 and ABI2. ABI proteins are a class of cytoplasmic molecular junction proteins originally identified as binding chaperones for Abl kinase. The ABI3 gene is thought to be an important regulator of microglia during Alzheimer's disease (AD), and its S209F variant increases the risk of developing AD. Moreover, ABI3 plays an important role in suppressing tumor growth and progression.

实验方案

Product Specific Protocols
WB protocol for ABI3 antibody 16227-1-APDownload protocol
IHC protocol for ABI3 antibody 16227-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols