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验证数据展示

  • WB analysis of mouse brain using 22433-1-AP

    WB analysis of mouse brain using 22433-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 22433-1-AP (ABLIM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 22433-1-AP

    IHC staining of mouse skeletal muscle using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22433-1-AP (ABLIM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 22433-1-AP

    IP experiment of mouse brain using 22433-1-AP

    IP result of anti-ABLIM2 (IP:22433-1-AP, 4ug; Detection:22433-1-AP 1:1000) with mouse brain tissue lysate 1280 ug.

  • IHC staining of human skeletal muscle using 22433-1-AP

    IHC staining of human skeletal muscle using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22433-1-AP (ABLIM2 Antibody) at dilution of 1:50.

  • IHC staining of mouse skeletal muscle using 22433-1-AP

    IHC staining of mouse skeletal muscle using 22433-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22433-1-AP (ABLIM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ABLIM2 Polyclonal antibody

ABLIM2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 22433-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

abLIM 2, ABLIM2, Actin binding LIM protein 2, KIAA1808

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse skeletal muscle tissue, human skeletal muscle tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22433-1-AP targets ABLIM2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ABLIM2 fusion protein Ag18198 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actin binding LIM protein family, member 2
别名 abLIM 2, ABLIM2, Actin binding LIM protein 2, KIAA1808
计算分子量 611 aa, 68 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC122567
基因名称 ABLIM2
Gene ID (NCBI) 84448
RRIDAB_11232422
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6H8Q1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABLIM2 antibody 22433-1-APDownload protocol
IHC protocol for ABLIM2 antibody 22433-1-APDownload protocol
IP protocol for ABLIM2 antibody 22433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols