验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, human heart tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 2 publications below |
产品信息
22673-1-AP targets STARS/ABRA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | STARS/ABRA fusion protein Ag18456 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin-binding Rho activating protein |
| 别名 | ABRA, Striated muscle activator of Rho-dependent signaling, STARS, Actin-binding Rho-activating protein |
| 计算分子量 | 381 aa, 43 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC105103 |
| 基因名称 | STARS/ABRA |
| Gene ID (NCBI) | 137735 |
| RRID | AB_11182712 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N0Z2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for STARS/ABRA antibody 22673-1-AP | Download protocol |
| IHC protocol for STARS/ABRA antibody 22673-1-AP | Download protocol |
| IF protocol for STARS/ABRA antibody 22673-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Development HAND2-mediated epithelial maintenance and integrity in cardiac outflow tract morphogenesis. | ||
Behav Brain Res Changes in mRNA and miRNA expression in the prelimbic cortex related to depression-like syndrome induced by chronic social defeat stress in mice | ||
Am J Pathol Different mouse models of nemaline myopathy harboring Acta1 mutations display differing abnormalities related to mitochondrial biology |
