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验证数据展示

  • WB analysis of mouse liver using 14148-1-AP

    WB analysis of mouse liver using 14148-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 14148-1-AP (ABT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 14148-1-AP

    IHC staining of human brain using 14148-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14148-1-AP (ABT1 antibody) at dilution of 1:100 (under 10x lens).

  • IF Staining of HeLa using 14148-1-AP

    IF Staining of HeLa using 14148-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ABT1 antibody (14148-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

ABT1 Polyclonal antibody

ABT1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 14148-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

hABT1, Esf2, Activator of basal transcription 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue, HeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14148-1-AP targets ABT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 ABT1 fusion protein Ag5317 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 activator of basal transcription 1
别名 hABT1, Esf2, Activator of basal transcription 1
计算分子量 31 kDa
观测分子量 31-32 kDa
GenBank蛋白编号BC066313
基因名称 ABT1
Gene ID (NCBI) 29777
RRIDAB_10640440
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULW3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ABT1 antibody 14148-1-APDownload protocol
IHC protocol for ABT1 antibody 14148-1-APDownload protocol
IF protocol for ABT1 antibody 14148-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

JCI Insight

ABT1 modifies SMARD1 pathology via interactions with IGHMBP2 and stimulation of ATPase and helicase activity

Authors - Gangadhar P Vadla
View Article