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ACAD10 Polyclonal antibody

ACAD10 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 17161-1-AP

Print datasheet

Synonyms

ACAD 10, ACAD10

验证数据展示

  • WB analysis of mouse spleen using 17161-1-AP

    WB analysis of mouse spleen using 17161-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17161-1-AP (ACAD10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas using 17161-1-AP

    IHC staining of human pancreas using 17161-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17161-1-AP (ACAD10 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human pancreas using 17161-1-AP

    IHC staining of human pancreas using 17161-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 17161-1-AP (ACAD10 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 17161-1-AP

    IF Staining of HeLa using 17161-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ACAD10 antibody (17161-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17161-1-AP targets ACAD10 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACAD10 fusion protein Ag10510 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acyl-Coenzyme A dehydrogenase family, member 10
Synonyms ACAD 10, ACAD10
Calculated Molecular Weight 26 kDa, 119 kDa
Observed Molecular Weight 119 kDa
GenBank Accession NumberBC015056
Gene Symbol ACAD10
Gene ID (NCBI) 80724
RRIDAB_2219552
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6JQN1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACAD10(Acyl-CoA dehydrogenase family member 10) belongs to the acyl-CoA dehydrogenase family. ACAD10 has significant activity towards the branched-chain substrates R and S, 2 methyl-C15-CoA and is highly expressed in fetal but not adult brain. This pattern of expression is similar to that of LCAD, another ACAD previously shown to be involved in long branched chain fatty acid metabolism(PMID:21237683). It has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for ACAD10 antibody 17161-1-APDownload protocol
IHC protocol for ACAD10 antibody 17161-1-APDownload protocol
IF protocol for ACAD10 antibody 17161-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols