验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, MCF-7 cells, mouse heart tissue, mouse brain tissue, mouse liver tissue, rat liver tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84202-1-RR targets ACAD9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | ACAD9 fusion protein Ag8414 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | acyl-Coenzyme A dehydrogenase family, member 9 |
别名 | |
计算分子量 | 69 kDa |
观测分子量 | 60-65 kDa |
GenBank蛋白编号 | BC007970 |
基因名称 | ACAD9 |
Gene ID (NCBI) | 28976 |
RRID | AB_3671758 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | Q9H845 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Acyl-CoA dehydrogenases (ACADs) are a family of mitochondrial enzymes catalyzing the initial rate-limiting step in the β-oxidation of fatty acyl-CoA. ACAD9 belongs to the group of ACADs. The deduced 621-amino acid protein has a calculated molecular mass of 68.8 kD. It has an N-terminal leader sequence, 2 conserved motifs shared by all ACAD family members, and a potential N-glycosylation site(PMID:12359260). Defects in ACAD9 are a cause of acyl-CoA dehydrogenase family member type 9 deficiency (ACAD9 deficiency).
实验方案
Product Specific Protocols | |
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WB protocol for ACAD9 antibody 84202-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |