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ACADM Monoclonal antibody, PBS Only

ACADM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A11G6

Cat no : 67742-1-PBS

Print datasheet

Synonyms

ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH

验证数据展示

  • WB analysis using 67742-1-Ig (same clone as 67742-1-PBS)

    WB analysis using 67742-1-Ig (same clone as 67742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 67742-1-Ig (same clone as 67742-1-PBS)

    WB analysis of rat liver using 67742-1-Ig (same clone as 67742-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

    IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

    IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67742-1-Ig (same clone as 67742-1-PBS)

    IF Staining of human liver cancer using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ACADM antibody (67742-1-Ig, Clone: 1A11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 67742-1-Ig (same clone as 67742-1-PBS)

    WB analysis of HSC-T6 using 67742-1-Ig (same clone as 67742-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

产品信息

67742-1-PBS targets ACADM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, rat, pig samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, rat, pig
Immunogen ACADM fusion protein Ag30620 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Synonyms ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH
Calculated Molecular Weight 421 aa, 47 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC005377
Gene Symbol ACADM
Gene ID (NCBI) 34
RRIDAB_2918511
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11310
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.