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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67742-1-Ig (same clone as 67742-1-PBS)

    WB analysis using 67742-1-Ig (same clone as 67742-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 67742-1-Ig (same clone as 67742-1-PBS)

    WB analysis of rat liver using 67742-1-Ig (same clone as 67742-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

    IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

    IHC staining of human liver using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67742-1-Ig (ACADM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67742-1-Ig (same clone as 67742-1-PBS)

    IF Staining of human liver cancer using 67742-1-Ig (same clone as 67742-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using ACADM antibody (67742-1-Ig, Clone: 1A11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

  • WB analysis of HSC-T6 using 67742-1-Ig (same clone as 67742-1-PBS)

    WB analysis of HSC-T6 using 67742-1-Ig (same clone as 67742-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67742-1-Ig (ACADM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67742-1-PBS in a different storage buffer formulation.

ACADM Monoclonal antibody, PBS Only

ACADM Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67742-1-PBS

产品说明书

CloneNo. 1A11G6

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat, pig

应用

WB, IHC, IF-P, Indirect ELISA

别名

ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67742-1-PBS targets ACADM in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, rat, pig samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 Human, rat, pig
免疫原 ACADM fusion protein Ag30620 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
别名 ACAD1, ACADM, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH
计算分子量 421 aa, 47 kDa
观测分子量42 kDa
GenBank蛋白编号BC005377
基因名称 ACADM
Gene ID (NCBI) 34
RRIDAB_2918511
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11310
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACADM, also named as MCAD, belongs to the acyl-CoA dehydrogenase family. This enzyme is specific for acyl chain lengths of 4 to 16. It catalyzes the reaction: Acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor. Defects in ACADM are the cause of medium-chain acyl-CoA dehydrogenase deficiency (MCAD deficiency). This protein can exsit as a dimer(PMID:8962055). This antibody is specific to ACADM.